Evidence from heterologous expression of glutathione S-transferases A and A1 of the plaice (Pleuronectes platessa) that their endogenous role is in detoxification of lipid peroxidation products   总被引：2，自引：0，他引：2  

Martinez-Lara E  Leaver M  George S 《Marine environmental research》2002,54(3-5):263-266

cDNA clones for glutathione S-transferases A (GST-A) and A1 (GST-A1) from plaice (Pleuronectes platessa) were expressed as N-terminally 6XHis tagged proteins in Escherichia coli and purified to homogeneity from Ni-NTA silica. GST-A was an efficient catalyst for conjugation of unsaturated alkenals derived from peroxidation of polyunsaturated fatty acids with the highest activity observed with trans-non-2-enal (8 micromol min(-1) mg(-1)). GST-A1 was a very efficient Se-independent glutathione peroxidase with an activity towards cumene hydroperoxide of 25 micromol min(-1) mg(-1). Although the enzymes exhibited moderately high activities towards the model substrate 1-chloro-2,4-dinitrobenzene (CDNB) they exhibited little or no activity towards other common prototypical xenobiotic substrates. Together with data for ontogeny, tissue distribution and inducibility of these enzymes, we contend that a primary function of these enzymes is protection from the harmful effects of lipid peroxidation products generated naturally or exacerbated by xenobiotic exposure.  相似文献   

Contamination of organotin compounds and imposex in molluscs from Vancouver,Canada     

Horiguchi T  Li Z  Uno S  Shimizu M  Shiraishi H  Morita M  Thompson JA  Levings CD 《Marine environmental research》2004,57(1-2):75-88

Gastropods and bivalves were collected at 15 sites at Vancouver and Victoria, Canada between 24 May and 7 June, 1999, to establish tissue concentrations of butyltin and phenyltin compounds, to record imposex symptoms in gastropods, and to assess the present status of organotin contamination around Vancouver. No neogastropods (such as Nucella lima) were found around Vancouver. Neogastropod populations could have been extirpated by severe TBT contamination in Vancouver, as relatively high concentrations of TBT were detected in tissues of Mytilus trossulus from Vancouver, and the neogastropods distributed in Vancouver might be sensitive to TBT. Recovery from imposex, however, was observed in neogastropod populations from three sites at Victoria and Mission Point. TBT contamination has continued around Vancouver, arising from continuous use of TBT in antifouling paints for vessels larger than 25 m in length; however, TBT has decreased around Victoria and Mission Point. Different patterns of TBT accumulation in tissue were observed among the bivalve species from Vancouver. The highest TBT concentration detected in Tresus capax suggested some possible adverse effects. TBT was the most predominant butyltin component in almost all bivalve specimens surveyed, suggesting a low rate of TBT metabolism. Phenyltin compounds were not detected in any molluscan specimens in this study.  相似文献   

Variations of antioxidant efficiency and presence of endosymbiotic diatoms in the Antarctic porifera Haliclona dancoi     

Regoli F  Nigro M  Chierici E  Cerrano C  Schiapparelli S  Totti C  Bavestrello G 《Marine environmental research》2004,58(2-5):637-640

The role of endosymbiotic diatoms as pro-oxidant stressors in porifera has been investigated in the Antarctic sponge Haliclona dancoi in which the presence of diatoms is influenced by marked seasonal variations during the austral summer. Both chlorophaeopigments and frustules were absent in sponge tissues sampled in early November at the beginning of the summer and increased from the mid of December with slightly shifted temporal trends. The efficiency of antioxidant defenses in the sponge showed a marked response to symbionts with clearly enhanced values corresponding to the peak of diatoms.  相似文献   

印度板块东缘蛇绿岩的特征及其构造意义     

Sengu.  S 童春雷 《海洋地质译丛》1991,(1):68-71

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应用C¹⁴测定浮游生物初级生产力的方法     

M.S.Doty  M.Oguri 《海洋与湖沼》1964,6(3):297-308

本文从单位时间和单位体积内通过光合作用转变为有机碳的重量出发,介绍了一个测定有机物初级生产力的新的海洋学方法。为了使对此感兴趣的人都能够参照他们各自的需要来应用或改进这一同位素定量方法,我们简短地对操作方法和历史作了一般的评价之后,还作了相当详尽的叙述。  相似文献   

预测未勘探地区资源的一种新地表地球化学方法试验     

Hvosl.  S 孙佰强 《海洋地质译丛》1996,(2):4-13

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封堵性断层圈闭：一个成熟油区的勘探思路     

Nybak.  S 陈颐亨 《海洋地质译丛》1992,(3):18-23

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日本岛弧和俯冲作用     

Uyeda  S 潘秋霞 《海洋地质译丛》1992,(4):41-46

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南极沿海伯尔顿盐湖双刺镰状水蚤盐度耐性的季节变化     

王自磐  D.S.埃思莱克  H.R.伯尔顿 《海洋学报》1987,9(6):760-765

双刺镰状水蚤Drepanopus bispinosus为南极沿海伯尔顿盐湖(Burton lake)中的越冬种类之一,其雌性性成熟大约于每年的初冬季节,在随后8个多月的生活史中,经历了一个盐度从33(5月份)到39(11月份)的环境变化.本文报道了该水蚤雌性成体对环境盐度适应的季节差异,动物在冬季的耐低盐能力较夏季强,从冬季到夏季,动物对盐度的适应范围明显地向高盐度方向偏移,这与环境盐度对动物的驯化有关.在低盐水中,动物的呼吸率增高,且夏季大于冬季,因而进一步证实了环境盐度的变化对动物盐度适应性的影响.  相似文献   

Oxidative stress in ecotoxicology: from the analysis of individual antioxidants to a more integrated approach     

Regoli F  Gorbi S  Frenzilli G  Nigro M  Corsi I  Focardi S  Winston GW 《Marine environmental research》2002,54(3-5):419-423

An integrate approach will be discussed for investigations on oxidative stress in xenobiotic toxicity. While the analysis of individual antioxidants is useful for their sensitivity and to understand the mode of action of a stressor, the integration with the analysis of the total antioxidant capacity provides a more holistic assessment of the overall biological significance of such variations. TOSC has a greater predictive value on the health condition of the organisms and allows to discriminate the different role of specific ROS in oxidative stress syndrome.  相似文献